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KMID : 0357319940290040311
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Journal of the Korean Society for Microbiology
1994 Volume.29 No. 4 p.311 ~ p.318
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Development and Detection of Nonradioactive Toxin DNA Probe Specific for Cholera Effect of Polyethylene Glycol and Centrifugal Force in Chlamydia trachomatis Infection to McCoy cell
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유천권
박재구/김호훈/박기덕
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Abstract
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Cholera toxin(CT) is the major pathogenic factor causing the watery diarrhea of cholera. A polymerase chain reaction(PCR) amplifies a 302-bp fragment of the cholera toxin A subunit gene was used to identify toxigenic V. cholerae. The amplicon
treated
with klenow fragment and polynucleotide kinase was cloned into PBs SK(+/-) was sequenced and labeled with digoxigenin. By using digoxigenin labeled DNA probe to identify CT-producing V. cholerae, we carried out colony hybridization. All
CT-producing
strains of V. cholerae 01 and V. cholerae 0139 hybridized with digoxigenin-labeled DNA probe, while nontoxigenic strains did not hybridize. LT producing E. coli did not show positive reaction in PCR and colony hybridization.
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KEYWORD
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